肝动脉热化疗栓塞术后患者血清新喋呤和T淋巴细胞rDNA转录活性的改变

Changes of serum neopterin and T-lymphocytes rDNA transcription activity in peripheral blood of patients with primary hepatic cancer after thermochemoembolotherapy

目的 研究原发性肝癌患者肝动脉热化疗栓塞术前后血清新喋呤和外周血T淋巴细胞rDNA转录活性的变化及其临床意义。方法 分别用双抗体夹心酶联免疫法(ELISA)和核仁形成区相关蛋白银染技术测定45例中晚期肝癌行肝动脉热化疗栓塞术治疗前后血清新喋呤和外周血T淋巴细胞rDNA转录活性的改变,并与健康对照组比较分析。结果 肝癌组治疗前新喋呤增高,外周血T淋巴细胞rDNA转录活性降低,与对照组比较有显著性差异(P<0.01);行肝动脉热化疗栓塞术后,患者血清新喋呤降低,外周血T淋巴细胞rDNA转录活性增高,治疗前后有显著性差异(P<0.05);应用免疫调节剂(r-IL-2和r-IFN)组较未用组血清新喋呤下降和外周血T淋巴细胞rDNA转录活性升高更显著。结论 检测原发性肝癌患者血清新喋呤和外周血T淋巴细胞rDNA转录活性的情况,有助于了解病人的免疫状况,肝动脉热化疗栓塞术能改善机体免疫功能,给予合理的过继免疫治疗能增强这种作用。

Objective To investigate the changes of serum neopterin and T-lymphocytes rDNA transcription activity in peripheral blood of patients with primary hepatic cancer (PHC) before and after thermochemoembolotherapy and eveluate their clinic significance. Methods The level of serum neopterin and T-lymphocytes rDNA transcription activity in peripheral blood of 45 patients with PHC before and after thermochemoembolotherapy were measured by ELISA and a cell image analysis of Ag-NORs respectively and compared them with that of healthy controls. Results In the patients with PHC before thermochemoembolotherapy, the level of serum neopterin was higher while T-lymhocytes rDNA transcription activity was lower than that of healthy controls ( P <0.01) .After thermochemoembolotherapy, neopterin declined while T-lymphocytes rDNA transcription activity elevated ( P < 0.05) .The effects of the group combined with immune therapy (r-IL-2 and r-IFN) was more obvious than that of the group without immune therapy( P < 0.05) . Conclusions Measuring neopterin and T-ymphocytes rDNA transcription activity is helpful to know the patients' immune state. The method of thermochemoembolotherapy can improve immune function of the patients with PHC and has more significant effects in the group combined with suitable immune therapy.