鼻咽癌旁上皮细胞核DNA含量分析

Analysis of DNA Content of Paracancerous Lesions in Nasopharyngeal Carcinoma

应用显微分光光度计测定15例鼻咽癌19处癌旁病变的上皮细胞核DNA含量并与浸润癌相比较。中、重度异型增生上皮细胞核DI及超过2.5c细胞的百分数处于单纯增生+轻度异型增生与浸润癌之间,3组DI及超过2.5c细胞的百分数差异显著。中、重度异型增生以非整倍体为主,其细胞核DNA含量组方图相似于浸润癌。从细胞核DNA含量角度来看,中、重度异型增生是重要的癌前病变。

The nuclear DNA content of 19 paracancerous epithelial lesions in 15 cases of nasopharyngeal carcinoma and corresponding invasive carcinomas was determined by microspectrophotometer from sections of paraffin—embedded tissues. The DI and percentage exceeding 2.5c of moderate+severe dysplasias were in between those of simply hyperplastic+midly dysplastic lessions and invasive carcinomas,the differences of DI and percentage exceeding 2.5c among the 3 groups were significant. DNA distributions of the moderate+severe dysplasia were aneuploid—predominant,and DNA histograms of moderate+severe dysplasias were similar to those of invasive carcinomas. We concluded that the moderate+severe dysplasias might be the important precancerous lesions from aspect of DNA content analysis.