DN-1号防治糖尿病肾病鼠进行性肾损伤

Protective effects of DN-1 on progressively renal damage in STZ-induced diabetic rats and their mechansim

目的 观察DN 1号 (黄芪、党参、丹参、大黄复方合剂 )对糖尿病肾病的治疗效果 ,并对其作用机制进行探讨。方法 采用STZ单剂 (6 5mg/kg)腹腔注射方法建立糖尿病大鼠动物模型。动物分为糖尿病肾病对照组、糖尿病肾病DN 1号治疗组和正常对照组。观察DN 1号对各组血糖、血脂、血肌酐、转化生长因子 β1(TGF β1)、白介素 8(IL 8)、2 4h尿微量白蛋白排泄率、肾脏病理的影响。结果 糖尿病肾病组 4～ 8周对尿蛋白排泄率、血脂、TGF β1、IL 8水平 ,肾组织中TGF β1的表达均高于正常对照组。肾组织光镜和电镜检查均出现早期糖尿病肾病典型的病理改变。DN 1号治疗组 8周对尿白蛋白排泄率、血脂、TGF β1、IL 8较未治疗组低 (P <0 .0 1或P <0 .0 5 )。肾组织中TGF β1的表达也较对照组低。血糖在治疗组和对照组无显著性差异。结论 DN 1号能减少糖尿病肾病的尿蛋白排泄 ,对肾损伤有保护和延缓作用。对糖尿病肾病损伤保护机制与DN 1号降低血TGF β1、IL 8分泌 ,减少肾组织中TGF β1的表达有关。

Objective To evaluate the protective effects and study mechanism of DN-1 (Astragalus pilose, dangshen, Danshen, Rhubarb) on diabetic glomerulopathy in STZ induced diabetic rats. Methods A rat of STZ induced glomerulopathy was used in study. Diabetic rats were divided into two groups: one group was treated with DN-1 (RDN-1), another group with water (RDN-C), in addition, normal rats was used as controls (RNS). Changes of serum TGF-β_1, IL-8. Glucose, triglyceride, creatinine concentration and 24h urinary albumin rate (UAR), TGF-β_1, expression levels of rend tissue in rats were measured. Investigations of renal tissue in rats by microscope and electron microscope were analysed.Results In forth and eighth week urinary protein excretion and the levels of serum IL-8, TGF-β_1,and expression of TGF-β_1 in renal tissue were obviously higher in diabetic glomernlopathy rats than controls. By microscope and electron microscope. The typical pathological changes were found in the early stage of diabetic glomerulopathy rats. In eighth week, UAR,TG and expression levels of TGF-β_1,IL-8 were significantly decreased in diabetic rats treated by DN-1 than those untreated.Conclusion DN-1 may attenuate renal damage by reducing the urinary protein expression in diabetic glomerulopathy rats. The protective mechanism on diabetic nephropathy of DN-1 may be due to DN-1's decreasing the serum TGF-β_1,IL-8 levels and expression TGF -β_1 in renal tissue.