D-核糖提取精制的初步研究

The Preliminary Study on the Separating Process of D-Ribose

依次对发酵液进行离心、除蛋白、脱色、脱盐、浓缩等处理 ,确定了草酸除蛋白的用量 ,利用NKD 1、D4 0 2 0和NKA 93种型号大孔吸附树脂进行脱色比较实验 ,发现NKD 1在相同流速、温度、体积下脱色效果、吸附率、再生率综合效果比较最好 ,利用 0 0 1× 7,2 0 1× 7阴阳离子串联树脂进行脱盐实验 ,确定了树脂的有效交换容量 ,同时提出了以Ca2 + 、Mg2 + 、Cl- 含量判断脱盐终点的方法。将糖液浓缩至 4 0 %以上后 ,浓缩液与标准D 核糖溶液的高效液相图谱比较 ,结果表明 ,所提取D 核糖纯度已达到 98%以上。

To obtain D-ribose, fermentation broth was treated with centrifugation, deproteinization, decolorization, desaltification and concentration. The optimal consumption of oxalic acid used for deproteinization was determined. Effects of three resins, NKD-1, D4020 and NKA-9 on decolorization were compared. The results showed that the best effects in decolorization and sugar recovery rate were achieved using NKD-1 under the same flow rate, temperature and volume. Series-wound ion-exchange resins with 001×7 and 201×7 were used for desaltification. The effective exchange capacity was determined. A method using the concentration of Ca 2+, Mg 2+ and Cl - to determine the end of desaltification was reported. HPLC showed that D-ribose solution concentrated to above 40% meets the standard requirement.