羟基致死船舶压载水微生物的生化影响研究

Studty on Biochemical Effect of Hydroxyl on Microorganism of Ballast Water

试验表明羟基比值浓度达到0 63mg/L左右时,足以杀死压载水微生物。在此浓度下也会引起微生物的糖、脂质、蛋白质、核酸、酶等发生强烈的化学反应。脂质过氧化物含量上升为原来的3倍;总蛋白质减少了33 4%;核酸中RNA、DNA分别减少了46 9%、77 0%;葡萄糖却增加了1倍,羟基破坏了多糖,使之降解为单糖;过氧化物酶含量减少了21 9%;超氧化物歧化酶减少了54 2%;分离微生物后海水中过氧化氢酶、超氧化物歧化酶分别减少了83 1%、33 2%,上述生化试验资料清楚表明了羟基对微生物三大基本分子(核酸、蛋白质、葡萄糖)、抗氧化酶系(过氧化酶、过氧化氢酶、超氧化物歧化酶)以及脂质均有强烈的破坏作用,这也是致死压载水微生物的主要原因。

Experimental results indicated that about 0.63 mg/L hydroxyl was enough to kill the microorganism of ship's ballast water,and caused a series of chemical reaction in saccharide,lipid,protein,nucleic acid,enzyme of microorganism.As a result,the degree of lipid peroxide goes up to 3 times.The total protein reduced to 33.4%,RNA to 46.9% and DNA to 77.0%.However the glucose was increased to 2 times since the amylose was degraded to monosaccharide using Hydroxyl.POD was decreased to 21.9%,SOD to 54.2%;CAT and SOD went down 83.1% and 33.3% respectively in seawater without the cell.Therefore,The hydroxyl radical has a strong destruction action to three kinds of key molecules(nucleic acid,protein,glucose),antioxidant enzyme system and lipid of cell membrane,which is a main reason to kill the microorganism ship's ballast water.