护肝饮对饮酒大鼠肝细胞形态变化的影响

Effect of Huganyin on Morphology of Liver of the Rats Following Exposure to Ethanol

目的:观察中药复方护肝饮对饮酒大鼠肝组织形态变化的影响,为临床诊治酒精性肝病(ALD)寻找有效药物。方法:用Wistar大鼠30只,随机分为正常组、实验模型组和中药治疗组,实验模型组与中药治疗组大鼠每天饮用20％的酒精,不饮水,中药治疗组从饮酒后第5周开始中药治疗。于第8周末处死大鼠,观察肝组织的形态变化。结果:光镜下实验模型组大鼠肝细胞脂肪沉积,气球样变性,肝细胞灶状坏死,并有Mallory小体和嗜酸性小体形成,间质增生,小叶间胆管增多,电镜下肝细胞膜系结构受到破坏。而治疗组大鼠的肝组织损伤均有不同程度的改善。结论:护肝饮具有调节肝细胞的物质代谢,减少肝细胞内脂肪沉积,清除自由基,抑制质脂过氧化损伤,稳定肝细胞膜结构的作用。

Objictive: To study the morphology of the liver and the effect with the Huganyin treatment. Methods: 30 adult Wistar rats were divided into three groups randomly:(1) normal control group and (2) experimental ALD group, (3)treatment group with Huganyin. The rats in group (2) and (3)were made to drink alcohol(20% ) instead of water. From the fifth week, rats in group (3) were infused the Huganyin decoction, while drinking alcohol. At the end of the eighth week of the experiment, all the rats were killed; the morphology of the liver were observed under light and electron microscope. Results: In group (2) there were a large number of fat vacuole which distributed around the nucleuses differed in size within the liver cell cytoplasm. Some edematous liver cells showed the balloonlike forms. Mallory and eosinophilic bodies were formed. The interlobukr bile ducts had increased and the hyperplasia of the interstitial cells ocoused. The structures of the cell membrane series were destroyed. Comparaed to group (2), in group (3), all the injuries were cured in some way. Conclusion: The Huganyin can adjust the metabolism of the liver cells; it has an effect on removing the free radical, controlling lipid peroxidation and the inflammatory reactions, and making the membranal structures more steady.