黄芪对感染血吸虫小鼠肝组织外周型苯二氮受体表达的影响

Effects of Huangqi on the Expression of PBR in Liver of Schistosomiasis Mice with Liver Fibrosis

目的:探讨黄芪对感染血吸虫小鼠肝组织外周型苯二氮受体表达的影响。方法:采用黄芪注射液腹腔注射,在小鼠感染血吸虫满8周开始治疗,疗程12周,与生理盐水作对照。应用实时监测荧光定量PCR技术,于感染满8周、12周和20周时检测肝组织外周型苯二氮革受体mRNA的表达水平。结果:黄芪治疗组小鼠外周型苯二氮受体mRNA水平在感染12周和20周时均明显低于同期对照组,与正常组接近;黄芪治疗组治前、治中和治后外周型苯二氮受体mRNA水平比较差异无统计学意义,而对照组治疗前后差异显著。结论:黄芪可降低血吸虫病小鼠肝组织外周型苯二氮革受体mRNA表达水平,提示黄芪可能通过此机制阻止血吸虫病肝损伤的发展。

Objective: To explore the effects of Huangqi on the expression of perpheral benzodiazepine receptor (PER) in liver of schistosomiasis mice. Methods : The mice which had been infected by schistosomiasis for 8w were treated with Huangqi for 12w. The changes of the content of PBRmRNA in liver of schistosomiasis mice after Huangqi treatment were observed by real-time FQ-PCR method. The results were judged with circle number of times (CT) when the level of amplification exponent achieved detecting liminal value. The detecting liminal value was the smallest level, at this time, the amplification exponent could be detected. The GT was inverse ratio to the level of exponent. Results: Comparing the Huangqi treated group (n = 8) with the saline control group (n= 8) at 12thw, 20thw, after infection, the change of Cr was significant. PBRmRNA expression was obviously lower in Huangqi treated group than that saline control group (12thw: 28.50vs 25.25, P<0.01; 20thw, 27.54vs 23.60, P<0.01); The level of PBRmRNA expression after Huangqi treatment were not obviously higher than that in 6 normal mice (12thw; 28.50vs 30.28, P<0.05; 20thw, 27.54vs 29.70, P<0.05). Conclusion: Huangqi could reduce the content of PBRmRNA significantly in liver of schistosomiasis mice. It suggested that Huangqi could restrain the development of the schistosomiasis by the PBR.