丁酸钠联合穿琥宁对人大肠癌细胞HCT-8增生的影响

Effect of sodium butyrate combined with chuanhuning on HCT-8 cell line proliferation

目的:探讨丁酸钠、穿琥宁体外联合应用对结肠癌细胞的影响。方法:应用生长曲线、MTT、流式细胞仪、电镜研究丁酸钠、穿琥宁对HCT-8的增生抑制及诱导凋亡作用。结果:丁酸钠对HCT-8细胞有明显抑制作用,48h后各组抑制率分别为15.7％,20.3％,33.3％(P<0.01),呈现浓度、时间依赖性。电镜下观察可见成熟分化改变及凋亡细胞,流式细胞仪可检测到亚G1峰,细胞周期分析发现其主要阻滞在G1期。穿琥宁与其联合应用,混合组1在24h,48h凋亡率为23.5％,48.6％,混合组2在24h,48h凋亡率为30.8％,54.2％(P<0.01)。结论:丁酸钠抑制HCT-8细胞增生,并诱导其成熟分化、凋亡。穿琥宁与其合用使凋亡率增加。

AIM: To study the effect of sodium butyrate in combination with chuanhuning'on HCT-8 cell line proliferation. METHODS: Inhibition of HCT-8 cell line by sodium butyric acid in combination with chuanhuning was detected by MTT assay and growth curve,and apoptosis was determined by morphological assay and flow cytometry (FCM).Apoptotic cells were observed electro- microscopically. RESULTS: Sodium butyric acid showed inhibitory effect on the proliferation of HCT-8 cell line in dose-dependent and time-dependent manner.The inhibitory rates were 15.7%, 20.3%,and 33.3%(P<0.01)in different groups.Differ- entiation and apoptosis were observed under electronic microscope.Sub-G1 peak was detected by FCM.Cell cycle was blocked in S phase.The apoptotic rate of combined group 1 were 23.5%,48.6% at 24h, and 48h, and the apoptotic rate of combined group 2 were 30.8%,54.2% at 24h,and 48h(P<0.01). CONCLUSION: Sodium butyric acid can induce apoptosis and differentiation of HCT-8 cells of human colorectal carcinoma, and inhibit proliferation of HCT-8 cells. Apoptotic rate was significantly increased when sodium butyric acid was combined with Chuanhuning.