细胞生长因子对冠状动脉内皮细胞增殖与迁移的影响

THE EFFECTS OF CELL GROWTH FACTORS ON PROLIFERATION AND MIGRATION OF CORONARY ARTERY ENDOTHELIAL CELLS

目的 :观察肝细胞生长因子 (HGF)和血管内皮细胞生长因子 (VEGF)对体外培养牛冠状动脉内皮细胞(BCAEC)增殖、迁移的影响。方法 :分离和培养BCAEC ,设对照组、VEGF组、HGF组。采用四甲基偶氮唑蓝法(MTT)观察细胞增殖 ;倒置显微镜观察培养的血管内皮细胞的迁移。结果 :对照组、VEGF组、HGF组的OD值分别为 0 .2 2± 0 .0 1、0 .40± 0 .1 4、0 .44± 0 .1 5 ;VEGF组、HGF组BCAEC的增殖率分别为 81 .8%± 1 6 .9%、1 0 0 %±2 1 .1 % ;对照组BCAEC迁移不明显 ,而VEGF组和HGF组BCAEC迁移明显。结论 :VEGF、HGF能促进BCAEC增殖、迁移 ,HGF作用强度不亚于VEGF。

Aim: To study the effects of hepatocyte growth factor(HGF) and vascular endothelial growth factor(VEGF) on proliferation and migration of bovine coronary artery endothelial cells(BCAEC) in vitro . Methods: BCAECs were isolated and cultured in vitro , and divided into control group,VEGF group and HGF group. BCACEs proliferation were measured using MTT, and their migration was observed using reverse microscope. Results: The OD value of control, VEGF and HGF group were 0.22±0.01?0.40±0.14? 0.44 ±0.15 respectively. The proliferation ratio of BCAECs in VEGF and HGF group was 81.8%±16.9%?100.0%±21.1% respectively. There was no migration in control group, but significant migration in VEGF and HGF group. Conclusion: Both VEGF and HGF can promote proliferation and migration of BCAECs, the effect of HGF is stronger than VEGF.