糖皮质激素对人绒毛膜滋养层细胞11β-HSD1的调节

GLUCOCORTICOIDS UP-REGULATE HUMAN CHORION 11β-HYDROXYSTEROID DEHYDROGENASE TYPE1

目的 :研究糖皮质激素对 1 1 β 羟基类固醇脱氢酶Ⅰ型 (1 1 β hydroxysteroiddehydrogenasetype 1 ,1 1 β HSD1 )还原酶活性和mRNA表达的调节。方法 :利用原代培养的人类绒毛膜滋养层细胞 ,运用免疫细胞化学染色方法、放射性酶活性测定及Northern印迹杂交技术 ,结合图像分析技术检测 1 1 β HSD1mRNA的表达量。 结果 :1 1 β HSD1和糖皮质激素受体 (glucocorticoidreceptor,GR)免疫活性样物质共存于原代培养的人绒毛膜滋养层细胞中 ,人工合成的糖皮质激素———地塞米松对绒毛膜滋养层细胞 1 1 β HSD1还原酶活性和mRNA表达具有诱导作用 ,此诱导作用可以被GR阻断剂RU486所阻断。结论 :糖皮质激素与GR结合后上调绒毛膜滋养层细胞 1 1 β

Aim: To study the co localization of glucocorticoid receptor and 11β hydroxysteroid dehydrogenase 1 (11β HSD1) and to investigate whether glucocorticoids regulate the reductase activity and expression of 11β HSD1. Methods: Immunohistochemical staining for 11β HSD1 in cultured primary human chorionic trophoblasts was performed. Radiometric conversion assay and Northern blot analysis were respectively used to observe the activity and mRNA expression of 11β HSD1. Results: 11β HSD1 and GR were co expressed in the same chorionic trophoblast. Both 11β HSD1 reductase activity and mRNA levels were increased by dexamethasone (10 -6 mol/L,10 -7 mol/L) in the cultured chorionic trophoblasts, and the effects were blocked by GR antagonist RU486 (10 -6 mol/L). Conclusion: By binding to GR, glucocorticoids induce the expression of 11β HSD1 by a possible intracrine mechanism.