发光二极管作为现场叶绿素荧光仪激发光源实验研究

Experimental Study on LED as the Excitation Resource of Fluorometer for the in situ Measurement of Chlorophyll-a Concentration in Water

本文介绍了超高亮蓝紫发光二极管(light emitting diodes,LED)作为一种新型激发光源,激发水体中叶绿素a发荧光。将LED与传统应用于水体的叶绿素荧光仪器光源进行比较.通过两种实验装置,测量了荧光信号,并且获取叶绿素a的荧光光谱,验证了LED作为叶绿素荧光激发光源的可行性,为研制现场连续走航叶绿素荧光仪提供了新的光源选择.

In this paper, we introduced the super-bright blue-purple light emitting diode (LED) into a novelty light resource which excited chlorophyll-a to emit fluorescence. Furthermore, we compared LED with the ordinary excitation resource. By two experiment apparatus, we have measured the fluorescence signal and obtained the chlorophyll-a fluorescence emission spectra. The results show that LED can act as an alternative excitation resource in developing the fluorometer for the in situ measurement of the chlorophyll-a concentration in water.