基因芯片在筛选宫颈癌相关基因中的应用研究

Preliminary study on the gene expression pattern in cervical cancer

目的 :通过研究宫颈癌组织和正常宫颈组织中差异表达的基因 ,筛选出与宫颈癌相关的基因群 ,并进一步研究其表达和初步功能。方法 :以含 2 0 4 8条人类全长基因的 c DNA基因表达谱芯片 ,检测 3例宫颈癌标本和正常宫颈标本的基因表达谱 ,计算机分析比较两种组织中差异表达的基因。 结果:3例宫颈癌差异表达基因分别有 197条、4 2 9条和 6 6 5条 ,共同差异表达的基因有 6 4条。 结论 :宫颈癌同其他肿瘤一样 ,是多种基因结构和功能改变的结果 。

Objective: To investigate the differentially expressed gene in cervical cancer and from those in normal cervical tissue and screen out some cervical cancer associated genes. Methods: Gene chips containing 2048 human genes were used to investigate the gene expression pattern of 3 samples of cervical cancer. The differentially expressed genes were selected when two patterns were compared using computer. Results: By applying this gene chip, differentially genes 197、429 and 665 were screened out respectively in 3 cervical cancer samples. 64 differentially expressed genes in all 3 cervical cancer samples were identified. Conclusion: Cervical cancer results from many changes of genes as the other malignant carcinomas. The specific cervical cancer related genes and their function need to be investigated further.