p53、PCNA和Bcl-2蛋白表达与胃癌发生的关系

The relationship between expression of p53,PCNA and Bcl-2 protein and the development and progression of the gastric carcinoma

目的:探讨 p5 3、Bcl- 2、PCNA蛋白表达在胃癌发生发展过程中的异常表达及其内在关系。方法:随机选取 14 2例胃粘膜炎性增生及轻、中重度异型增生和胃癌活检组织标本 ,切片分别进行 HE染色病理诊断和免疫组化 L SAB法检查 p5 3、PCNA、Bcl- 2蛋白表达。 结果 :轻度不典型增生和炎性增生中无 p5 3表达 ,PCNA、Bcl- 2阳性表达率由炎性增生、异型增生到癌变逐渐增高 ,胃癌的表达率显著高于良性病变 (炎性增生、轻度异型增生 ) ( P<0 .0 5 ) ,p5 3、Bcl- 2蛋白阳性表达在胃癌与中重度异型增生间无统计学差异 ( P >0 .0 5 )。 结论 :胃癌前病变即有Bcl- 2蛋白的高表达 ,p5 3基因过度表达是胃粘膜癌前病变的晚期标志之一。在胃癌的发生发展过程中 ,p5 3、Bcl- 2基因发挥着既独立又协同的作用 。

Objective: To study the relationship and significance between multiplication and apoptosis of gastric carcinoma. Methods: 142 cases of various gastric lesion tissues, including inflammatory hyperplasia, mild dysplasia, moderate and severe dysplasia and gastric carcinoma, were studied by immunohistochemistry using monoclonal anti p53, anti PCNA and Bcl 2 antibodies. All data were treated by χ\+2 and related biostatistic analysis. Results: The detected rates of positive expression of p53 protein, PCNA and Bcl 2 protein revealed significant difference between the gastric malignant (carcinoma) and the benign lesions (hyperplasia and mild dysplasia) (P<0.05), but there was no statistic difference between the carcinomatous lesion and severe dysplasia lesion in positive expression of p53, Bcl 2 protein (P>0.05). Conclusions: The development and progression of the gastric carcinoma, may have an abnormal alteration of cellular genes, e.g. those genes controlling cell multiplication and apoptosis. The p53 gene overexpression is relatively late event in stomach tumorigenesis. Bcl 2 protein expression alteration might play a role in the early development. p53 and Bcl 2 gene play both independent and synergetic role in the course and development of gastric carcinoma. The severe dysplasia lesions may have the biopathologic character of carcinoma.