4种药物对大鼠肝纤维化防治作用的实验研究

Preventive effect of malotilate、salvia milti、PHGF and hepadif on liver fibrosis of rats

目的 :验证丹参、马洛替酯、促肝细胞生长素 (以下简称促肝 )和疗尔健 4种药物对大鼠实验性肝纤维化的防治作用 ,并探索各药物间可能存在的协同或拮抗作用。 方法:采用正交设计法 ,用大剂量 CCl4 在短期内诱导大鼠肝硬化 ;以大鼠肝组织纤维化病理分期作为疗效判定指标 ,验证 4种药物对肝纤维化的防治作用。用正交设计方差运算 ,以各因素的 F值作为判断各药物差异统计学指标。结果:马洛替酯组大鼠肝纤维化程度较轻 ,中心静脉区及汇管区可见纤维组织增生 ,但肝小叶结构基本完整。其余治疗组虽未形成假小叶 ,但正常肝小叶已被破坏 ;对照组则形成典型假小叶。结论 :马洛替酯对 CCl4 所致大鼠实验性肝纤维化有防治作用 ( P <0 .0 5 ) ;丹参、促肝、疗尔健对 CCl4 所致大鼠实验性肝纤维化还不能认为有防治作用 ( P >0 .0 5 ) ;4种药物在肝纤维化防治方面不存在协同或拮抗作用 ( P >0 .0 5 )。

Objective: Orthogonal design was used to study preventive effect on experimental fibrosis of liver and the possible interactive effect of malotilate、 salvia milti、 PHGF and hepadif in hepatic fibrosis of rats in order to provide experimental basis for clinical treatment of liver fibrosis. Methods: With large dose of carbon tetrachloride, liver fibrosis were induced in rats. Orthogonal design was used in the experiment. Each factor's mean square value of orthogonal variance analysis was adopted as the distinguished indication. Results: In malotilate therapeutic groups, the hepatic histologic structures were basically complete, moderate proliferating fibrosis were found. In other therapeutic groups, strong fibrosis and many lobules were separated, however, the typical pseudolobule was not yet found. In control group, lobules of liver were destroyed in most areas, in which they were replaced by fibrosis and pseudolobular structure. Conclusions: Malotilate has significant fibrosis preventive effect (P<0.05). No significant fibrosis preventive effect was found in salvia milti、 PHGF or hepadif (P>0.05). The experimental result showed that no antagonal or cooperation was found among the four kinds of medicine (P>0.05).