聚乙烯醇包埋固定黄孢原毛平革菌方法的探讨

Probing of the Entrapment Method for Phanerochaete chrysosporium with PVA

以PVA为包埋剂对黄孢原毛平革菌进行固定,并建立包埋颗粒对染料比布列希猩红的反应体系。表明:PVA浓度为10%较适合;交联剂H3BO3的pH为6 7有利于成球;海藻酸钠的加入可防止粘连发生;复合包埋、冷冻法、Al2(SO4)3处理小球或在培养基中添加CaCO3等,均能或提高小球的机械强度或促进降解反应,但有时也会引起负面效应。

Phanerochaete chrysosporium was entrapped with PVA and the reaction system for dye Biebrich Scarlet with entrapment particles was set up.The study showed:the appropriate concentration of PVA was 10%; pH67 of crosslinking agent H3BO3 was of benefit to the forming of beads; the addition of sodium alginate could prevent agglomeration;the complex-supports-entrapment,the freezing mehthod,the treatment of beads with Al2(SO4)3 or the supplemnt of CaCO3 in culture medium ect.could increase the strength of beads or promote the degrading of dye,but it was possible that they also caused some negative effects.