正交法筛选超声提取银杏果中黄酮及多糖的工艺

Orthogonal Test for Ultrasound-assisted Extraction of Flavonoids and Polysaccharides from Ginkgo Biloba Fruit

目的:筛选银杏种仁中具有抗氧化作用的银杏果多糖和黄酮的超声优化提取工艺。方法:通过正交实验法来确定银杏果多糖和黄酮超声辅助提取法的最优条件。采用硫酸-苯酚法确定多糖含量;三氯化铝显色法确定总黄酮的含量。结果:最优提取工艺为料液比:1:30,超声时间:30min,温度:40℃,其中,料液比、超声时间、温度对提取率的影响依次减小。在此条件下得到的总提取率为12.27%。

OBJECTIVE:To optimize the ultrasound-assisted extraction of polysaccharides and flavonoids from Ginkgo biloba fruits.METHODS:The optimum conditions of ultrasound-assisted extraction of polysaccharides and flavonoids from Ginkgo biloba fruit were determined by orthogonal experiment.The content of polysaccharides was determined by sulfuric acid-phenol method and the content of total flavonoids was determined by aluminium trichloride chromogenic method.RESULTS:The optimum condition was as follows:solid-liquid ratio:1:30,ultrasonic time:30 min,temperature:40℃.The effects of solid-liquid ratio,ultrasonic time and temperature on extraction rate decreased in turn.Under this condition,the total extraction rate was 12.27%.