摘要
为研究汉滩病毒对肿瘤细胞的诱导凋亡作用,以一定量病毒悬液感染体外培养的SP2/0细胞,接种后一定时间将细胞消化甩片行Gimsa染色观察凋亡细胞核的变化,制细胞悬液以流式细胞仪测细胞周期,并用免疫组化的方法检测凋亡分子Fas和FasL的表达。结果示经病毒诱导后细胞出现生长特性及形态学变化,Giemsa染色观察到典型凋亡细胞;流式细胞仪显示有凋亡峰出现;免疫组化检测出感染后SP2/0细胞中Fas和FasL表达明显升高。该结果表明汉滩病毒可诱导体外培养SP2/0细胞凋亡,其发生可能与凋亡分子Fas和FasL有关。
To study the apoptosis of malignant carcinoma cells induced by Hantaan virus, SP2/0 cells cultured in vitro were infected with Hantaan virus. Their apoptosis profiles were detected using Giemsa staining, flow cytometry and immunohistochemistry staining. Giemsa staining showed typical apoptotic changes in infected cells; Apoptosis peaks were detected by flow cytometry; Immunohistochemistry staining showed apparent increase of Fas and FasL expression in infected cells compared with normal cells. The results suggest that Hantaan virus could induce SP2/0 cells in vitro to undergo apoptosis which might be associated with Fas and FasL.
出处
《中国病毒学》
CSCD
2003年第4期315-317,T001,共4页
Virologica Sinica
