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HCV核心蛋白诱导Cos-7细胞凋亡 被引量：1

HCV Core Protein Induces Apoptosis in Cos-7 Cell Line
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摘要 将丙型肝炎病毒(HCV)核心蛋白(Core)基因的全长序列插入到真核表达载体pCDNA3 CMV启动子下游,构建真核表达载体pCDNA3-Core,用脂质体LipoVec^(TM)转染Cos-7细胞系进行瞬时表达;DNA转染24h后用免疫斑点试验检测在细胞中表达的Core蛋白;转染72h后用Hoechst染色和DNA Ladder检测Cos-7细胞的凋亡情况;荧光染色观察到了细胞凋亡核碎裂,琼脂糖凝胶电泳也呈现出180-200bp整数倍的梯形带,呈现典型的细胞凋亡特征。这些结果表明HCV Core蛋白的表达能引起Cos-7细胞凋亡,Core蛋白的这种功能可能在HCV的持续感染过程中起着一定的作用。 Recombinant plasmid pCDNA3-Core was constructed by inserting Hepatitis C virus(HCV-1) core gene into the site between EcoR Ⅰ and Xba Ⅰ of eukaryotic expression vector pCDNA3. The recombinant plasmid pCDNA3-Core was transfected to Cos-7 cell line by LipoVec^(TM). Expression of core protein was detected by immunology Dot blot 24h after transfection. The nuclear cracks in transfected cell was observed by Hoechst 33258 staining and the 180-200bp DNA ladders also was detected 72h after transfection. These results reveal that HCV Core protein can induce apoptosis of Cos-7 cell and its function may play an important role in HCV chronic and persistant infection.
作者 佘应龙 叶林柏 廖庆姣 叶力 郭泰林 杨晓骏
机构地区 武汉大学生命科学学院病毒研究所
出处 《中国病毒学》 CSCD 2003年第4期326-329,T002,共5页 Virologica Sinica
基金 国家自然科学基金(30070175)
关键词 HCV 核心蛋白 细胞凋亡 丙型肝炎病毒 Hepatitis C virus(HCV) Core protein Apoptosis
分类号 R373 [医药卫生—病原生物学]
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参考文献16

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同被引文献25

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中国病毒学
2003年 第4期

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