影响DpCPV杀虫剂高产条件的研究

Studies on Affected Factors of High Output of Dendrolimus punctatus cypovirus Insecticide

DpCPV杀虫剂的产量不仅受到气候、环境等因素的影响,而且严重受到生产工艺技术水平的制约.针对DpCPV杀虫剂生产过程中工艺技术问题,我们对马尾松毛虫质型多角体病毒湖南株在室内、室外和林间大量增殖中的集虫虫质、接种浓度、频率、收获时间等及其相互间的关系进行了详细研究,得出在DpCPV杀虫剂生产过程中接种1×107CPB/mL病毒浓度为宜,接种虫龄以4～5龄虫为宜,收获病毒时间13～14d为宜.其室外单虫病毒产量平均可达到7.5×108CPB,比国内报道要高.研究结果对DpCPV杀虫剂的高产增殖有很强的指导意义.

Not only the output of insecticide DpCPV is always affected by climate and circumstances, but also affected by the technology of production highly. Studies have been done on DpCPVHn indoors and outdoors for the Dendrolimus punctatus quanlity of every collection and the inoculation concentration and the inoculation frequency and the harvest time. The optimal conditions of multiplication have been gained. The optimal inoculation concentration is 1×10~7CPB/mL .The optimal Dendrolimus punctatus larvae for multiplication are 4 or 5 instar larvae. The optimal CPV ingathering time is13th or 14th day after inoculation. The outdoor yield of each larva can be 7.5×10~8CPB .It is higher than other reports in China. It is very important to guide high output of DpCPV multiplication.