小白鼠乳腺上皮细胞的体外培养

In vitro culture of mammary epithelial cells in mice

本试验对小白鼠乳腺上皮细胞的原代培养方法进行了探索。胶原酶消化法和组织块种植法均可用于培养小白鼠原代乳腺细胞。胶原酶消化法容易得到均匀的乳腺细胞 ,且上皮细胞所占比例也比较高。组织块种植法不容易丢失、损伤上皮细胞 ,然而上皮细胞长出较晚 ,且占比例较低。结果表明 ,可结合组织块转移。

The methodology of primary culture of mammary epithelial cells in micewas studied in this experiment. Both collagenase digestion and tissue cultivation could be used in culturing mammary epithelial cells. By collagenase digestion, well-proportioned mammary cells were easily obtained with a higher percentageof epithelial cells. By tissue cultivation, epithelial cells were well kept andprotected, but grew out late with a lower percentage. It was shown that epithelial cells could be purified by both tissue transfer and collagenase digestion