亚低温预处理大鼠局灶性脑缺血的保护作用

Experimental study on the preconditioning therapy for protection against focal ischemia in rats by mild hypothermia

目的 :模拟神经外科手术缺血过程 ,研究亚低温预处理情况下大鼠局灶性脑缺血的耐受时间。方法 :采用线栓法制作可逆的大鼠大脑中动脉缺血再灌注模型。 72只SD大鼠随机分成 6组 :1h、2h、3h缺血 常温组 ,1h、2h、3h缺血 亚低温预处理组。术后评价大鼠的神经功能以及脑梗塞体积的变化。结果 :对于 1h和 2h缺血组 ,应用亚低温预处理 ,可以改善大鼠的神经功能恢复、减小脑梗塞体积。对于 3h缺血组 ,则脑保护作用不明显。结论 :应用亚低温预处理大鼠局灶性脑缺血 ,可以减轻脑缺血性损害 ,脑缺血的耐受时间应在 2h以内。

Objective:To simulate the ischemia of neurosurgery and study the duration of brain ischemia in rats by preconditioning therapy of mild hypothermia Methods: Reversible middle cerebral artery occlusion (MCAO) was induced by employing a intraluminal suture SD rats were assigned randomly to one of six groups (n=12) as follows:1 hr,2hrs,3hrs MCAO normothermic group and 1 h, 2h,3h MCAO mild hypothermia group Neurological examinations, infarcted volume Results:In 1 h and 2h MCAO groups, applying mild hypothermia before the ischemia can improve the neurological outcome and reduced the infarcted volume In 3h MCAO mild hypothermia groups, there is no effect of brain protection Conclusion: Preconditioning therapy of mild hypothermia can reduce the injury of brain ischamia, the duration of brain ischemia is within 2hrs