双孢蘑菇丛生变异的RAPD分析及差异片段的克隆

RAPD Analysis of the Clustering Variation of Agarucus bisporus and Cloning of the Different DNA Fragment

通过双孢蘑菇正常菌株及其丛生变异菌株基因组DNA的RAPD研究,找到一个与双孢蘑菇丛生变异可能相关的DNA片段,将其克隆并测序.GenBank查询结果表明该片段可能的部分开放读框(ORF)与多种生物的二氢硫辛酰胺脱氢酶(DLDH)的部分氨基酸序列有较高的同源性,由此提出了一个双孢蘑菇丛生变异的分子机理假设.

A DNA fragment possibly related to the clustering variation of A.bisporus was found by RAPD of the genomic DNA of healthy strains and their clustering variation ones, and it was cloned and sequenced. The results of GenBank searching suggested that the possible partial ORF of the fragment had a high homology with part of amino acids of dihydrolipoamide dehydrogenase (DLDH) from several organisms, so a molecular mechanism model for the clustering variation of A.bisporus was supposed.