大鼠各组织中和PC12细胞中神经限制性沉默因子(NRSF)全长转录本的检测及部分序列的克隆

Detection of NRSF in All Kinds of Tissues of Rat and PC12 and Cloning of Some of Its Sequence

检测了大鼠的心、肝、肾、脑组织和不同培养条件下的PC12细胞中的NRSF全长转录本,了解到NRSF全长转录本存在于以上所有组织和细胞中,该基因受到转录后加工水平的调控,并且成功将NRSF基因片段IV整合到大肠杆菌基因组中,为今后对该基因的进一步研究奠定了基础.

The Present work is to detect the whole length transcriptional product of NRSF in heart, liver, kidney, brain of rat and the PC12 cell cultured under different conditions. The result indicated that the whole length transcription product of NRSF existed in all above these tissues and cells, and the regulation to this gene occurred at posttranscription level. The segment IV of NRSF gene was integrated into genome of E.coli successfully. The result of experiment Would be helpful to future research of this gene.