摘要
以黄曲霉 (Aspergillusflavus )为出发菌株 ,经 3次紫外线、 1次 60 Co、 3次亚硝基胍多重复合诱变处理 ,选育获得曲酸生产菌UCN7- 1 7,配以最佳培养条件 ,发酵 7d,曲酸产量由原来的 0 92 6% ,提高到 6 3%。实验证明采用多因子复合诱变 ,能有效改变菌株对诱变因素敏感性 ,提高变异率 。
mutant (UCN 7 17) of producing high yield Kojic acid was screened fr om Aspergillus flavus after treated with UV three times, γ ray of 60 Co one time and NTG four times, underoptimal conditions, the Kojic aci d production level reached up to 6 3% after 7 days, compared with original stains 0 926% The experiments showed that compound mutation using various mutagenic agents ca n alter the original stains sensitivity to mutagenic agents, increase mutation frequency and raise Kojic acid yield
出处
《微生物学通报》
CAS
CSCD
北大核心
2003年第4期60-66,共7页
Microbiology China
基金
陕西省重大攻关课题资助项目 (No 970 4 10 1)
关键词
曲酸
复合诱变
突变株
Kojic acid, Compound mutation, Mutant
