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RNA剪接因子结构与功能研究进展 被引量：4

Structural Analyses of RNA Splicing Factors: Progress and Perspective
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摘要 RNA剪接是一个多步骤、形成多种中间状态复合物的复杂过程 .尽管在已经发现的一百多种 pre mRNA剪接相关因子中 ,仅研究了约 8%相关蛋白质的空间结构 ,已充分显示对剪接相关因子三维晶体结构以及溶液结构的测定与研究 。 Pre-mRNA splicing is a critical step in eukaryotic gene expression and regulation. The removal of introns occurs in spliceosome, a macromolecular machine containing small nuclear ribonucleoproteins (snRNP), heterogeneous nuclear ribonucleoproteins (hnRNP) and K-homologous domain (KH) proteins. Crystallographic and NMR studies have begun to provide insight into the architecture of snRNP and the structural basis for RNA-protein as well as protein-protein interactions involved in pre-mRNA splicing. Elucidating molecular mechanisms underlying pre-mRNA splicing awaits systematic structural analyses of splicing factors and complexes.
作者 吴瑛 赫荣乔
机构地区 中国科学院生物物理研究所 Washington University
出处 《生物化学与生物物理进展》 SCIE CAS CSCD 北大核心 2003年第4期503-508,共6页 Progress In Biochemistry and Biophysics
基金 国家基金委纳米生物学重大计划重点项目 (90 2 0 60 41) 中国科学院创新方向性项目 (KSCX2 SW 2 14 )~~
关键词 RNA剪接因子 结构 功能 snRNA结合蛋白 hnRNA结合蛋白 剪接体 晶体结构 RNA splicing snRNA binding protein hnRNA binding protein spliceosome structure RNA binding domain
分类号 Q756 [生物学—分子生物学]
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参考文献28

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二级参考文献10

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生物化学与生物物理进展
2003年 第4期

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