摘要
RNA剪接是一个多步骤、形成多种中间状态复合物的复杂过程 .尽管在已经发现的一百多种 pre mRNA剪接相关因子中 ,仅研究了约 8%相关蛋白质的空间结构 ,已充分显示对剪接相关因子三维晶体结构以及溶液结构的测定与研究 。
Pre-mRNA splicing is a critical step in eukaryotic gene expression and regulation. The removal of introns occurs in spliceosome, a macromolecular machine containing small nuclear ribonucleoproteins (snRNP), heterogeneous nuclear ribonucleoproteins (hnRNP) and K-homologous domain (KH) proteins. Crystallographic and NMR studies have begun to provide insight into the architecture of snRNP and the structural basis for RNA-protein as well as protein-protein interactions involved in pre-mRNA splicing. Elucidating molecular mechanisms underlying pre-mRNA splicing awaits systematic structural analyses of splicing factors and complexes.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2003年第4期503-508,共6页
Progress In Biochemistry and Biophysics
基金
国家基金委纳米生物学重大计划重点项目 (90 2 0 60 41)
中国科学院创新方向性项目 (KSCX2 SW 2 14 )~~