蘑菇基肥中真菌多样性的研究

Study on the Fungal Diversity in Mushroom Compost

应用 18SrDNA同源性分析和常规微生物培养方法 ,对蘑菇基肥中的真菌种群进行了研究 .采用纯培养技术分离基肥中的真菌 ,机械破壁法提取分离真菌总DNA ,真菌特异引物对EF4 /EF3扩增18SrDNA ,并进行测序及序列相似性比较 ,结果表明 :纯培养技术分离自基肥中的真菌种属较少 ,从 11株分离菌中只鉴定获得 2株不同真菌Chaetomiumelatum和Penicilliumexpansum .18SrDNA部分片段 2次聚合酶链反应 (PCR)扩增及温度梯度凝胶电泳 (TGGE)分析结果表明 :不同时间取自同一地点的原始蘑菇基肥样本中的真菌种群结构要比纯培养技术的分析结果复杂得多 .研究结果表明 。

The fungal communities in mushroom compost was assessed using a combination of PCR amplification and sequencing of partial 18S rDNA from fungal isolates and “nested' PCR-TGGE analysis on the basis of DNA directly extracted from compost samples. The diversity of cultivated fungi isolated from compost samples was relatively low. A total of 11 isolates were related to only 2 different species. One species, Chaetomium elatum , was identified within 10 isolates, and the other, with high similarity belonged to Penicillium expansum. The fungal flora associated with mushroom compost was then monitored with “nested' PCR-TGGE. The patterns obtained revealed the more complex existence of fungal communities from the original compost samples than one from enriched with food waste.