β-环糊精葡萄糖基转移酶高产菌株02-5-71的选育及发酵条件研究

STUDY ON THE SCREENING AND FERMENTATION OF CYCLODEXTRIN GLUCANOTRANSFERASE HIGH-PRODUCTION STRAINS

应用氮离子注入对嗜碱芽孢杆菌进行诱变育种,获得了产生β-环糊精葡萄糖基转移酶是出发菌株2倍以上的高产菌株,酶活力可达6000U/mL以上.应用正交实验法筛选出最优发酵条件.

The cyclodextrin glucanotransferaseproducing strain was treated with nitrogen ionbeam injection. A mutant was obtained and its enzyme productivity was above 6 000 U/ mL ,that was 2fold higher than that of parent strain. The best fermentative condition was selected using orthogonal test .