HPLC法测定依那普利的血药浓度

Determination of the concentration of enalapril in plasma by HPLC

目的建立高效液相色谱法测定马来酸依那普利的血药浓度。方法采用SpherisorbC18柱(2 5 0mm× 4 6mm ,5 μm) ,流动相 :乙腈 -甲醇 -水 -磷酸 -三乙胺 (V∶V∶V∶V∶V =5 0∶2 3 0∶2 75∶0 3∶0 1 ) ,流速 :0 9mL·min-1,检测波长 2 1 5nm。结果马来酸依那普利的血药浓度在 1 0～3 0 0 μg·L-1内 ,与其峰面积有良好的线性关系 (r=0 9942 )。方法回收率为 1 0 1 1 % ,日内精密度小于 5 5 % ,日间精密度小于 5 4%。结论该方法可用于马来酸依那普利的药代动力学及生物利用度研究。

Objective To establish a method for the determination of the concentration of enalapril maleate in plasma by HPLC. Methods Analytical column was Spherisorb C 18,the mobile phase consisted of acetonitrile,methanol,water,H 3PO 4,triethylamine(50∶230∶275∶0 3∶0 1,V∶V∶V∶V∶V).The flow rate was 0 9 mL·min -1.The detection was performed at UV215nm.Results The calibration curve was linear in the concentration ranged from 10 to 300 μg·L -1( r=0 994 2).The detection limit was 10 μg·L -1 and the recovery was 101 1%.The RSD within a day was less than 5 5% and the RSD between days was less than 5 4%.Conclusion The method is accurate and can be used for studying the pharmacokinetics and bioavailability of enalapril maleate. This method was applied to the pharmacokinetic analysis of enalapril in dogs.