用组培法培养马铃薯—倍体和双单倍体进行染色体加倍的研究

CHROMOSOME DOUBLING IN MONOHAPLOID AND DIHAPLOID POTATOES BY REGENERATION IN VITRO FROM CULTURED LEAF AND STEM SEGMENTS

分别取来自普通栽培种(S.tuberosum)的两个一倍体品系和两个双单倍体品系的茎段和叶片,用两步法进行组织培养来加倍其体细胞染色体数目。在以MS+2.25mg/1 BAP+5mg/1 NAA 为第一步的培养基上,从一倍体和双单倍体的茎段和叶片中获得了许多再生植株,其中约60%的加倍成植株(2n=2x=24或2n=4x=48),此结果表明此法是把单单倍体(以下简称一倍体)和双单倍体转化为纯合同源四倍体的有效方法。

Two monohaploid clones and two dihaploid clones derived from Solanum tuberosum were tested for doubling of their chromosome number by placing leaf pieces and stem seg- ments on media in two steps.More regenerated plants were produced from both monohaploid and dihaploid leaf and stem segments on medium containing MS+2.25 mg/l BAP+5 mg/l NAA in first step.The percentage of doubled plants(2n=2x=24 or 2n=4x=48)was about 60% in both monohaploid and dihaploid regeneration plants.This result suggests that it is a useful method to convert monohaploid and dihaploid into homozygous tetraploid potatoes.