狼疮性肾炎肾组织CD134共刺激信号对核因子-κB表达的影响

Expression and role of CD134 and NF-κB in renal tissue of lupus nephritis

目的 :探讨CD134(OX4 0 )对核因子 -κB(NF -κB)表达的影响及CD134和NF -κB在狼疮性肾炎 (LN)发病机制中的可能作用。方法 :对 4 0例LN患者肾组织 ,用免疫组织化学方法检测CD134的表达 ,用碱性磷酸酶 -小鼠抗碱性磷酸酶免疫复合物法 (APAAP)测定NF -κB表达 ,分析CD134与NF -κB的相关性。结果 :LN患者肾组织中 ,CD134表达显著上调 ,NF -κB在肾小球及肾小管也呈高水平表达 ,尤其以Ⅳ型LN更为明显。除系膜细胞、内皮细胞和远曲小管CD134表达明显增强外 ,肾间质毛细血管和大血管内皮细胞亦有CD134表达阳性的浸润细胞。LN患者肾小球和肾小管中CD134与NF -κB的表达呈正相关 (r分别为 0 5 5 4 2 ,0 6 2 79;P <0 0 5 ,P <0 0 5 )。结论 :LN患者存在CD134共刺激分子的异常表达 ;通过CD134分子的异常信号通路的活化 ,NF -κB在LN肾组织局部的表达明显增高 。

AIM: To investigate the role of CD134 (OX40) and NF-κB in the pathogenesis of lupus nephritis (LN). METHODS: Renal in situ CD134 and NF-κB expression were examined in 40 biopsy specimens from LN patients by immunohistochemistry and microwave-based immunohistochemistry, respectively. The relationship between expression of CD134 and NF-κB was analyzed. RESULTS: The expression of glomerular and tubular CD134 and NF-κB in LN were higher than that in normal control, especially in class Ⅳ LN, where there was intense staining of endothelial cell, distal tubules, and interstitial mononuclear cell. The CD134 expression of glomerular and tubular was closely related to NF-κB expression, respectively ( r=0.5542,P<0.05;r=0.6279,P<0.05). CONCLUSIONS: The abnormal expression of costimulatory molecule CD134 was well evidenced in LN. Strong expression of renal in situ NF-κB was likely mediated by CD134 signal pathway, which may play an important role in the pathogenesis of LN.