益骨胶囊含药血清对大鼠破骨细胞凋亡和分泌抗酒石酸酸性磷酸酶的影响

Effect of Yigu capsule drug-containing serum on apoptosis and tartrate resistant acid phosphatase secretion in rat osteoclasts

目的 :观察益骨胶囊含药血清对体外培养的大鼠破骨细胞 (OC)分泌抗酒石酸酸性磷酸酶 (TRAP)和凋亡的影响。方法 :( 1)将 2 0只 12月龄SD大鼠随机分为益骨胶囊灌胃组和生理盐水灌胃组 ,制备含药血清和对照血清 ;( 2 )分离培养新生SD大鼠OC ,加血清培养后 ,重氮盐法检测TRAP ,倒置显微镜下观察OC存活数及荧光染色法观察OC凋亡情况。结果 :与空白血清组比较 ,含药血清组在 2 4h、4 8h和 72h时TRAP的活性均明显降低 ,OC存活数明显降低 ,凋亡比率明显高 (P <0 0 1或P <0 0 5 )。结论 :益骨胶囊含药血清可以抑制体外培养OC分泌TRAP ,诱导OC凋亡 。

AIM: The effects of Yigu capsule on tartrate resistant acid phosphatase (TRAP) secretion and apoptosis in rat osteoclasts were investigated in order to further explore its mechanism of preventing and treating osteoporosis. METHODS: (1) Twenty-month-old Sprague-daweley rats were randomly divided into two groups(Yigu capsule group and saline group), and the drug-containing serum and control serum were prepared. (2) The newborn Sprague-daweley rat osteoclasts were cultured with different concentrations of Yigu capsule drug-containing serum. At different time point, TRAP activity was measured and the survival osteoclast was counted under reverse microscope.The percentage of osteoclast apoptosis was observed under fluorescence microscope after acridine orange staining. RESULTS: TRAP activity was lower and the percentage of osteoclast apoptosis was higher in drug-containing serum group than in control group at 24, 48 and 72 h( P <0 01),respectively, and the survival osteoclasts were less in drug-containing serum group than in control groups at 24, 48 and 72 h( P <0 01). CONCLUSIONS: These data suggest that Yigu capsule drug-containing serum induces apoptosis and inhibits TRAP activity in osteoclasts, which may be one of the mechanisms of Yigu capsule preventing and treating osteoporosis.