后基因组时代基因功能分析的策略

Strategies of Functional Genes Analysis in the Post-geneomic Era

人类基因组计划的完成标志着生命科学已经开始迈进后基因组时代 ,大规模基因的功能研究正成为生命科学研究的热点。基因功能的分析方法日趋成熟和多样化 ,综述了基因敲除、反义技术、“dominantnegative”策略 (“显性失活”策略 )、基因诱导超表达、RNA干涉以及生物信息学等基因功能分析策略的特异性及其优缺点。

The achievement of the human genome planning (HGP) brings bioscience into the post-genomic era. Functional analysis of new genes becomes the highlight of the bioscientific researches. Strategies of functional genes analysis become more advanced and more diversified. This review is mainly related to the basic principle, specificity, advantages and disadvantages of several main strategies of gene functional analysis including gene knock-out, antisense technic, “dominat negative' strategy , gene inducible overexpression, RNA interference and bioinformatics, et al. ;