一种简便、快速从病毒感染鱼、虾组织中制备PCR模板的方法

A Simple and Rapid Method to Prepare the Template for PCR Amplification from the Virus-infected Tissues of Fishes and Shrimps

以虹彩病毒 (iridovirus)感染大黄鱼的脾组织、对虾白斑杆状病毒 (whitespotsyndromebaculovirus,WSBV)感染中国对虾的肌肉组织为材料 ,采用一种简便、快速的方法获得了可满足病毒PCR检测的高质量模板DNA ,分别以针对虹彩病毒、WSBV的特异性引物进行PCR扩增 ,均能有效扩增出预期的条带。与常规DNA病毒模板制备方法相比 ,具有简便、快速、提取率高、无污染等优点 ,尤其适用于水产动物病毒PCR检测试剂盒的商品化开发及生产实际应用。

A simple and rapid method to prepare the templates for PCR detection of the viruses from aquatic animals was reported in this paper. The highly - qualified templates were obtained quickly from spleens of iridovirus - infected large yellow croakers and muscular tissues of the white spot syndrome baculovirus (WSBV) - infected shrimps by this method, and the expected fragments were amplified effectively from these two templates with the specific primers for iridovirus and WSBV detection, respectively. This method has advantages of simplicity, rapidness, safety and high - effectivity of extraction, compared with the conventional phenol - chloroform extraction. So it is very applicable to the commercial exploitation of PCR detection kit and practical utility. ;