大豆异黄酮和钙对大鼠成骨细胞增殖分化和矿化的作用

Effects of soybean isoflavone and calcium on proliferation, differentiation and mineralization of cultured osteoblasts in vitro

为探讨大豆异黄酮和钙对体外培养大鼠颅骨成骨细胞增殖、分化和矿化的作用 ,体外培养新生大鼠颅骨成骨细胞 ,然后用噻唑盐 (MTT)法测定大豆异黄酮和葡萄糖酸钙对体外培养成骨细胞的增殖作用 ,氨基安替比林测酚法测成骨细胞内碱性磷酸酶 (ALP)活性 ,茜素红染色方法 (ARS)研究对成骨细胞矿化的影响。结果单纯补钙组大鼠成骨细胞增殖、分化和矿化无显著促进作用。大豆异黄酮可显著地促进成骨细胞的增殖和分化 ,并促使成骨细胞形成矿化结节 ,而补充大豆异黄酮同时补钙可使矿化结节团块增大。大豆异黄酮可以提高成骨细胞的增殖和分化 。

To explore the effects of soybean isoflavone and calcium on proliferation, differentiation and mineralization of cultured osteoblastic cells. Osteoblastic cells from newborn rat calvarial was cultured. Osteoblastic cells proliferation was measured by MTT, activity of APL was observed with Golden's method and ARS was used to measure the mineral nodes of osteoblasts. The results showed that the calcium group had not different frome the control group on stimulating the proliferation, differentiation and mineralization of rat calvarial osteoblastic cells. The soybean isoflavone group had significant effects on stimulating the proliferation, differentiation of osteoblasts and increase the forming of the mineral nodes of osteoblasts in rat. Calcium and soybean isoflavone group had more benefit for forming mineral nodes. The soybean isoflavone stimulates the proliferation, differentiation and mineralization of rat calvarial osteoblastic cells, and increase calcium in the same time is more benefit for mineralization.