氨基酸对热暴露果蝇生存能力的影响

Effects of amino acids on the survival of heat-exposed Drosophila

为研究氨基酸对热暴露果蝇生存能力的影响 ,筛选对热损伤有防护能力的有效氨基酸。选取黑腹果蝇 (Drosophilamelanogaster) ,野生型Oregonk品系 ,在 2 5℃普通培养基中生长、繁殖 ;收集 1 0h内在基础培养基羽化的成虫 ,区分雌雄 ,分为对照组及实验组 ,基础培养基适应 2 5～ 3天后 ,将实验组移入添加氨基酸的培养基 ,对照组继续在基础培养基 2 5℃饲养。实验组果蝇在 36 5℃热环境饲养 ,1 0h后每隔 2h计数果蝇死亡数 ,直到果蝇全部死亡。共计筛选了 1 8种氨基酸 ,其中果蝇在添加了 5种氨基酸的培养基中热暴露平均存活时间明显高于对照组 ,这 5种氨基酸是谷氨酰胺、苯丙氨酸、酪氨酸、谷氨酸和精氨酸 ;其它 1 3种氨基酸没有明显延长热暴露果蝇生存时间的效果。提示谷氨酰胺、苯丙氨酸、酪氨酸、谷氨酸和精氨酸可以延长热暴露果蝇生存时间 ,但不存在剂量

To investigate the effects of amino acids on the survival of heat exposed Drosophila and to select the amino acids that can protect the Drosophila from heat exposure, Drosophila melanogaster were fed with basic culture in 25℃. The male virgin Drosophilas were distinguished from female. Both male and female virgin Drosophilas were divided respectively to the trail groups and control group with randomization. The trail groups were fed with culture supplying different amino acids in 37 5℃. After 10 hours, the dead insects were counted every two hours until all of them were dead. The mortality rates of heat exposed Drosophilas that feed in the culture added with the glutamine, arginine, phenylanaline, tyrosine and glutamic acid were obviously higher than that of control. The glutamine, arginine, phenylanaline, tyrosine and glutamic acid might increase survival time of heat exposed Drosophila, but a dose response relationship was not observed.