HPLC法测定阿司匹林咀嚼片中游离水杨酸含量

Determination of free salicylic acid in chewing aspirin tablets by HPLC

目的建立HPLC法测定阿司匹林咀嚼片中游离水杨酸含量的方法.方法以十八烷基硅烷键合硅胶为填充剂,甲醇-水-冰醋酸(8.0:5.5:1.0)为流动相测定阿司匹林咀嚼片中游离水杨酸含量(检测波长302nm).结果该法具有良好的分离度,被测溶液稳定,水杨酸在2.65～31.77μg/ml(r=0.999 97)的浓度范围内线性关系良好;此方法平均回收率为100.21%,相对标准偏差(RSD)为0.53%(n=6).结论本方法快速、准确、稳定,适用于测定阿司匹林咀嚼片中游离水杨酸的含量.

Objective To establish a HPLC method for determining the content of free salicylic acid in chewing aspirin tablets. Method The determination was conducted on a HPLC column (C 18 , 150 mm×4.6 mm×5 μm) with methanol-water-glacial acetic acid (8.0∶5.5∶1.0) as the mobile phase and the detection wavelength of 302 nm. Results The calibration curve was lin-ear within the concentration range of 2.65 to 31.77 μg/ml (r=0.999 97) of salicylic acid. The average recovery rate was 100.21% with relative standard deviation of 0.53% (n=6). Conclusion HPLC is quick and accurate of determining the content of free salicylic acid for chewing aspirin tablets.