组织工程新材料蜘蛛拖丝蛋白的重组培养

Recombinant culture of spider dragline silk protein as a new tissue engineering material

目的通过对重组蜘蛛拖丝蛋白基因工程菌pNS2生长的培养基进行优化,为获取大量蜘蛛拖丝蛋白提供条件。方法采用单因子试验和正交试验对培养基进行优化。结果获得优化的培养基组成:葡萄糖5.0g/L、蛋白胨5.0g/L、磷酸二氢钾(KH2PO4)15.0g/L、微量元素母液15.0g/L、硫酸镁(MgSO4)0.8g/L、柠檬酸1.7g/L。培养基优化后,工程菌的生长量提高了60.6%。结论优化的培养基为基因工程菌pNS2大规模生产蜘蛛拖丝蛋白奠定了基础。

Aim To optimize the medium for the growth of genetic e n -gineering Escherichia coli pNS2,pr oviding a nice condition for the mass cul ture of spider dragline silk proteins.Methods The single-factor experi-ments and the orthogonal experiment s were adopted to opitimize the medi-ums.Result s The optimized culture medium consis ted of 5.0g /L Glucose,5.0g /L Pe ptone,15.0g /L KH 2 PO 4 ,15.0g /L Trance elements storeso-lution,0.8g /L MgSO 4 ,1.7g /L Citric acid.The growth of en gineering bacteria increased 6 0.6%in the optimized culture.Conclusion The opti-mized culture medium is u sed for genetic engineering Escherichia coli pN S2to produce the recombinant sp ider dr agline silk protein in a large scale.