亚力克对小鼠脑损伤后还原型谷胱甘肽的影响:随机正交试验

Influences of ALEC on reduced glutat hione after mouse brain injury: a randomized and orthogonal trial

目的亚力克(ALEC)是由α-亚麻酸(A)、L-赖氨酸(L)、维生素E(E)及维生素C(C)组成的复方,通过正交试验设计,以脑组织中乳酸含量为指标,确定4者比例(ALEC为432187188)。研究ALEC对小鼠脑损伤后还原型谷胱甘肽(GSH)及谷胱甘肽过氧化物酶(GSH-PX)和超氧化物歧化酶(SOD)的影响。方法采用小鼠急性持续性缺氧脑损伤模型。结果小鼠经持续性低氧处理后,脑组织中GSH含量及GSH-PX、SOD活性明显降低(P<0.01,t=2.5665～6.9902,2.2195～8.1458,1.4817～12.2546),缺氧前给予ALEC高、中、低3个剂量,均可增加脑组织中的GSH含量及活性。其中高、中剂量组作用明显,低剂量组可增加脑组织中GSH含量,但无统计学意义。结论持续性低氧脑损伤可致脑组织中GSH含量及GSH-PX、SOD活性降低,ALEC可对抗这种下降作用。

Aim ALEC i s a complex consisting of(α-linolenic acid(A),L-lysine(L),Vitamin C(C )and Vitamin E(E),and their proportion was deduced by orthogonal experimen t with the content of lactic acid in br ain as the index,which showed the optima l proportion should be ALEC =432187188.This trial aimed to study the effect of ALEC on reduced glutathione(GSH),glutathione peroxidas e(GSH-PX)and superoxide dis-mutase(SOD)after mice brain injury.Methods I n this experiment,59mice were randomly divided into five groups:normal salin e group,Nimodi pine group,high dose of ALEC group,middl e dose of ALEC group and low dose of ALEC group,and then underwent continuous hypoxia.Results The GSH content,SOD and GSH-PX activities i n brain tissue were significantly re- duced(P<0.01,t=2.5665-6.9902,2.2195-8.1458, 1.4817-12.2546).Mice pretreated with three levels o f ALEC showe d an obvious increase in the content of GSH and the activities of SOD and GSH-P X.And the high and middle dose of ALEC showe d a significant effect on the cont ent of GSH and the activities of SOD and GS H-PX.Conclusion ALEC inhibits the depletion of GSH and the attenuation of SOD and GSH-PX activities in-duced by continuous hypoxia.