异体羊膜复合神经生长因子桥接长段周围神经缺损的可行性研究(英文)

Study on peripheral nerve deficit bridged by amnion allograft combined with nerve growth factor

目的探讨同种异体羊膜材料复合应用神经生长因子替代神经材料桥接周围神经缺损的可行性。方法48只健康SD大鼠制备坐骨神经缺损模型,随机分为4组,即自体神经原位移植组(A组),异体神经移植应用环孢素A(CyclosporineA,CSA)5mg/(kg.d)5周组(B组),异体羊膜材料桥接缺损复合应用神经生长因子组(C组),单纯异体神经移植组(D组)。6只Wistar大鼠取双侧坐骨神经作为供体。于术后12周取移植段神经行光镜、电镜形态学观察,测定神经运动诱发电位潜伏期、神经运动诱发电位峰值、神经传导速度和振幅积分、腓肠肌最大收缩力,再生轴突计数及髓鞘厚度测定。结果12周时A、B、C组的各项检测指标明显优于D组(潜伏期、诱发电位峰值、传导速度、腓肠肌最大收缩力、髓鞘厚度及再生轴突计数ANOVA结果分别为F=12.87,P<0.05;F=19.54,P<0.05;F=35.21,P<0.01;F=56.33,P<0.01.F=75.26,P<0.05;F=14.83,P<0.05;F=96.11,P<0.01),3组间差异无显著性(P>0.05),再生神经形态与功能恢复良好。结论对于长段周围神经缺损,应用异体羊膜复合神经生长因子进行桥接可以有效促进神经再生及功能恢复。

Aim To investigate the feasibili ty of using amnion allograft combined with nerve growth factor to bridge periphera l nerve deficit.Methods 48SD rats as models of sciatic nerve d eficit were ra ndomly assigned into one of the following groups:autograft(Group A),allograf t with Cyclosporine A(CSA)5mg /(kg·d)for 5weeks(Group B),deficit brid ged by amnion allograft combined with nerve growth factor(Group C),and allogr aft with no immune treatme nt (Group D).6Wistar rats were sacrificed as the donors of the sciatic nerve allograft.Observation was carried under light micro scope and e lectron microscope examination at 12weeks post-operatively for mo rphological studying;The latency,ampli-tude,conduct velocity and negative ar ea under the curve were recorded as electrophysiologic index;Maxim um contracti lity of gastrocnemius muscle and thickness of myelin of the regenenative nerve w ere measured;Axons counting were also performed t o evaluate the quality of reg enera-tion.Results At 12weeks,group A,B and C recovered better(Indexes su ch as latency,amplitude,conduct velocity ,negative area under the curve,maxi mum contractility of gastrocnemius muscle,thickness of mye lin and axons counti ng were compared by A NOVA test,F=12.87,P<0.05;F=19.54,P< 0.05;F=35.21,P<0.01;F=56.33,P<0.01.F=75.26 ,P<0.05;F=14.83,P<0.05;F=96.11,P<0.01),and no significant difference was f ound among these groups(P>0.05).Conclusion F or long distance peripheral nerve deficit,using amnion allograft combined wit h nerv e growth factor to bridge the deficit can facilitate the regeneration of nerv e and the recovery of function.