同型半胱氨酸诱导培养的人脐静脉内皮细胞分泌白细胞介素8

Homocysteine Induces Secretion of Interleukin-8 in Cultured Human Umbilical Vein Endothelial Cells

为探讨同型半胱氨酸对内皮细胞分泌白细胞介素 8的影响 ,及叶酸和牛磺酸对同型半胱氨酸作用的影响 ,将人脐静脉内皮细胞培养于不同浓度同型半胱氨酸中 ,采用酶联免疫吸附试验测定培养基中白细胞介素 8水平。结果发现 ,培养于同型半胱氨酸 (0 .1mmol L)中 4h、6h、8h和 1 2h ,白细胞介素 8分泌水平分别是对照组的1 .85倍、1 .88倍、2 .2 2倍和 1 .5 6倍 (P <0 .0 1 ) ;在不同浓度 (0 .0 5mmol L、0 .1mmol L、0 .5mmol L和 1mmol L)同型半胱氨酸中培养 8h ,各组内皮细胞白细胞介素 8的分泌水平分别是对照组的 1 .4 3倍、2 .1 6倍、2 .5 7倍和 2 .88倍(P <0 .0 5或P <0 .0 1 ) ,并呈剂量依赖性 ;同型半胱氨酸与叶酸 (0 .0 5mmol L)或牛磺酸 (5mmol L)共同培养细胞 ,各组内皮细胞白细胞介素 8的分泌水平无显著差异 (P >0 .0 5 )。以上结果提示 ,病理浓度的同型半胱氨酸能刺激内皮细胞分泌白细胞介素 8增加 ,这可能是同型半胱氨酸致动脉粥样硬化的机制之一 ;

Aim To investigate the effect of homocysteine on interleukin 8 production in endothelial cells, and the effect of folic acid and taurine on the role of homocysteine. Methods Human umbilical vein endothelial cells were incubated with homocysteine at a same concentration for different time or at increasing concentrations but for a same duration, and were incubated with homocysteine and folic acid or taurine for different time, then the interleukin 8 protein in the culture medium were determined by enzyme linked immunosorbent assay (ELISA). Results Enzyme linked immunosorbent assay showed that exposure of cells to homocysteine (0.1 mmol/L) for 4, 6, 8 and 12 hours, resulted in a 1.85 fold, a 1.88 fold, a 2.22 fold and a 1.56 fold increase respectively in interleukin 8 production, compared with the control group (P<0.01); meanwhile, exposure of cells to homocysteine at different concentrations (0.05 mmol/L, 0.1 mmol/L, 0.5 mmol/L and 1 mmol/L) for 8 hours, resulted in a 1.43 fold, a 2.16 fold, a 2.57 fold and a 2.88 fold increase respectively in secretion of interleukin 8, compared with the control group (P<0.05 or P<0.01 ); however, exposure of cells to homocysteine (0.1 mmol/L) and folic acid (0.05 mmol/L) or taurine (5 mmol/L) for different time, resulted in no signifcant difference in interleukin 8 production between groups. Conclusion Homocysteine induces secretion of interleukin 8 in cultured endothelial cells; and this role can be inhibited by folic acid and taurine.