甲基莲心碱对兔离体脑基底动脉收缩的影响

Effeccts of Neferine on the Rabbit Basilaris Arteria Constraction in Vitro

为了研究甲基莲心碱对兔离体脑基底动脉收缩的影响及与血管内皮的关系 ,采用离体家兔脑基底动脉环实验方法 ,以苯肾上腺素为兔脑基底动脉环的预收缩剂 ,测定血管张力变化 ,观察甲基莲心碱对血管收缩张力的影响 ;测定血管内皮完整组和去血管内皮组的张力变化 ,观察甲基莲心碱对血管收缩张力的影响是否为血管内皮依赖性的作用。结果发现 :甲基莲心碱抑制苯肾上腺素引起的兔脑基底动脉环收缩的作用与生理盐水对照组比较差异显著 (P <0 .0 0 1 ) ;甲基莲心碱对内皮完整组和对去内皮组血管环的最大舒张百分值分别为 38.4 %± 9.1 %和33.4 %± 1 1 .5 % ,差异无显著性统计学意义 (P >0 .0 5 )。提示甲基莲心碱能抗苯肾上腺素诱导的兔离体脑基底动脉收缩 。

Aim To study the effects of neferine (Nef) on constraction of the rabbit basilaris arteria Induced by phenylephrine (Phe). Methods The blood vessel tension responses to drug were measured by rabbit basilaris arteria rings (RBAR) endothelium being removed and intact endothelium in vitro. Results The RBAR precontracted with Phe can be relaxed by Nef with a significant difference from saline control group (P<0.001). The vasodilatation of Nef was not significantly differences between the endothelium removed and intact endothelium groups (P>0.05). Conclusions The RBAR precontracted with Phe can be relaxed by Nef in vitro. The vasodilatation of Nef is independent of endothelium.