脂多糖对胎儿主动脉平滑肌细胞合成基质金属蛋白酶1和基质金属蛋白酶抑制物1的影响

The Effect of Lipopolysaccharide on the Matrix Metalloproteinase-1 and Tissue Inhibitor of Matrix Metalloproteinase-1 in Fetal Aortic Smooth Muscle Cells

为探讨脂多糖是否通过影响基质金属蛋白酶 1和基质金属蛋白酶抑制物 1的平衡对动脉粥样硬化的进程起作用 ,应用体外培养的胎儿主动脉平滑肌细胞 ,加入不同浓度的脂多糖孵育 72h ,收集细胞上清液 ,用蛋白质印迹分析方法检测细胞上清液中基质金属蛋白酶 1和基质金属蛋白酶抑制物 1的含量。结果发现 ,脂多糖组与对照组相比 ,基质金属蛋白酶 1的含量明显减少 (P <0 .0 5 ) ,基质金属蛋白酶抑制物 1的含量没有显著差别。以上提示 ,脂多糖能抑制胎儿主动脉平滑肌细胞合成基质金属蛋白酶 1 ,但对基质金属蛋白酶抑制物

Aim To study whether lipopolysaccharide(LPS) regulates the production of matrix metalloproteinase 1(MMP 1) and tissue inhibitor of matrix metalloproteinase 1 (TIMP 1) in fetal aortic smooth muscle cells. Methods Aortic smooth muscle cells from 4～6month healthy abortive fetuses were incubated for 72 h with various concentration of LPS in vitro (0, 5, 10, 25 mg/L). The concentration of MMP 1 and TIMP 1 in the concentrated culture media was measured by Western Blotting. Results Compared with the control group, LPS could inhibit the production of MMP 1 in the smooth muscle cells, but could not change the production of TIMP 1 in the smooth muscle cells. Conclusions LPS could not promote the rupture of the atherosclerotic plaques by destroying the balance of the MMP 1 and TIMP 1 in the smooth muscle cells.