低密度脂蛋白、氧化型低密度脂蛋白、高密度脂蛋白和氧化型高密度脂蛋白对THP-1单核细胞ABC1 mRNA表达的影响

The Effects of Low Density Lipoprotein, Oxidized Low Density Lipoprotein, High Density Lipoprotein, Oxidized High Density Lipoprotein on the Expression of ABC1 mRNA in THP-1 Cells

为探讨低密度脂蛋白、氧化型低密度脂蛋白、高密度脂蛋白、氧化型高密度脂蛋白对THP 1单核巨噬细胞ABC1表达的影响 ,用 4 0 μg L佛波酯作用 4 8h ,将单核细胞诱导成巨噬细胞 ,再分别与 2 0mg L、1 2 0mg L的低密度脂蛋白、氧化型低密度脂蛋白、高密度脂蛋白、氧化型高密度脂蛋白作用 2 4h ,提取细胞中总RNA ,用逆转录聚合酶链反应法检测其ABC1mRNA的表达。结果发现 ,小剂量的低密度脂蛋白、氧化型低密度脂蛋白和氧化型高密度脂蛋白可引起THP 1细胞ABC1mRNA不同程度的表达增高 ,小剂量的高密度脂蛋白对ABC1表达无明显影响 ;而大剂量的低密度脂蛋白、氧化型低密度脂蛋白、高密度脂蛋白和氧化型高密度脂蛋白可使THP 1细胞ABC1mRNA的表达不同程度降低 ,其中高密度脂蛋白组轻微降低。结果提示 。

Aim To study the effect of low density lipoprotein (LDL), oxidized low density lipoprotein (ox LDL), high density lipoprotein (HDL), oxidized high density lipoprotein (ox HDL)on the expression of ATP binding cassette transporter 1 (ABC1) mRNA in THP 1 cells. Methods Cultured THP 1 cells were induced into macrophage by 40 μg/L phorbol ester (PMA)for 48 h and then incubated with 20 mg/L and 120 mg/L LDL, ox LDL, HDL and ox HDL for 24 h, total RNA of THP 1 cells were abstracted, reverse transcription polymerase chain reaction (RT PCR) were performed to inspect of the expression of ABC1 mRNA. Results The expression of ABC1 mRNA in THP 1 cells increased mildly in the presence of low dose of LDL, ox LDL and ox HDL, and low dose of HDL showed almost no effect on ABC1 expression; on the contrary, the expression of ABC1 mRNA decreased obviously in the presence of high dose of LDL, ox LDL and ox HDL, while HDL caused a mildly decrease in ABC1 mRNA expression in THP 1 cells. Conclusions Low dose of LDL, ox LDL and ox HDL can increase the expression of ABC1 mRNA in THP 1 cells; while high dose of LDL, ox LDL, HDL and ox HDL suppress ABC1 mRNA expression in THP 1 cells. This may imply that the effect of different levels of lipoproteins and oxidized lipoproteins on ABC1 expression play an important role in atherogenesis.