冠心病患者血管紧张素转化酶基因多态性与其血清活性及血管紧张素Ⅱ水平的关系

Association between Angiotensin Converting Enzyme Gene Polymorphism and Angiotesin Converting Enzyme Activity and Angiotensin-Ⅱ Level in Coronary Artery Disease

为探讨冠心病患者血管紧张素转化酶基因多态性与血清中血管紧张素转化酶活性及血浆中血管紧张素Ⅱ水平的关系 ,测定 76例冠心病患者和 6 8例健康人血管紧张素转化酶基因型、血清中血管紧张素转化酶活性和血浆血管紧张素Ⅱ水平 ,并对循环中血管紧张素转化酶活性和血管紧张素Ⅱ水平进行直线相关分析。结果发现 :①冠心病组和正常对照组血清血管紧张素转化酶、血浆血管紧张素Ⅱ水平无明显差别 ;②两组内不同基因型间血管紧张素转化酶活性存在显著差别 ,缺失型者明显高于杂合子和插入型者 ,但不同基因型间血管紧张素Ⅱ水平无明显差别 ;③血清血管紧张素转化酶活性和血浆血管紧张素Ⅱ水平无显著相关。结果提示 ,基因插入缺失多态性是决定血清血管紧张素转化酶活性的重要因素 ,但与血浆血管紧张素Ⅱ水平无关。血清血管紧张素转化酶活性和血浆血管紧张素Ⅱ水平无显著相关 ,血管紧张素转化酶基因多态性与冠心病发生。

Aim To study the association between angiotensin converting enzyme (ACE) gene polymorphism and ACE activity,angiotensin Ⅱ (AngⅡ) in coronary artery disease patients. Methods The serum ACE activity and plasma AngⅡ level in 76 patients with coronary artery disease and 68 healthy subjects were measured. The relation between serum ACE activity and plasma AngⅡ level was analyzed by linear regression. Results The serum ACE activity and plasma AngⅡ level were similar in coronary artery disease and control groups. But the serum ACE activity in different genotypes was significantly different in the two groups. The serum ACE activity in DD genotype (homozygotes for the deletion) was markedly higher than in ID genotypes (heterozygotes) and II genotypes (homozygotes for the insertion). The plasma AngⅡ level was similar in different genotypes in the two groups. No correlation was found between serum ACE activity and plasma AngⅡ level. Conclusion The ACE gene insertion/deletion polymorphism is the important factor that influenced the serum ACE activity. However it has no influence on plasma AngⅡ level. There is no relationship between the serum ACE activity and plasma AngⅡ level. The findings suggest the mechanism that ACE insertion/deletion polymorphism contribute to the generation or development of coronary artery disease is not completed by increasing plasma AngⅡ level.