摘要
目的 探讨多次缺血预处理 (IPC)对兔脊髓缺血再灌注损伤的保护作用及其机制。方法 2 4只日本大白兔随机双盲分为假手术组 (A组 )、缺血再灌注组 (B组 )和IPC保护组 (C组 ) ,每组 8只。A组不阻断主动脉 ,B组阻断主动脉 4 5min ,C组阻断主动脉 5min ,开放 5min ,反复 4次之后再阻断 4 5min。术后第 7天检测脊髓组织金属元素 (Ca、Mg、Cu、Zn)的浓度。术后观察后肢神经功能的评分、后肢针电极肌电图(EMG)和脊髓组织病理学的改变。结果 B组脊髓组织Ca、Cu的浓度较A组显著性升高 (P <0 .0 5或0 .0 1) ,Mg、Zn的浓度则显著性降低 (P <0 .0 5 )。B组脊髓组织Ca、Zn的浓度分别较C组显著性升高或降低(P <0 .0 1)。B组后肢神经功能评分均显著性低于A、C组 (P <0 .0 5或 0 .0 1) ,脊髓病理学和后肢EMG亦较C组有显著性病理改变 (P <0 .0 1)。结论 多次IPC对兔脊髓缺血再灌注损伤具有显著而又快速的保护作用 ,其保护机制与维持缺血区域Ca、Mg、Cu、Zn元素的平衡有关。
Objective To study the protective effects of multi ischemia preconditioning(IPC) on spinal cord ischemic injury and its mechanism in rabbits. Methods Twenty four rabbits were randomly divided into sham operated group (group A, n =8), ischemia reperfusion group (group B, n =8) and IPC group (group C, n =8). In group A, abdominal aorta wasn't crossclamped. Spinal cord ischemia was induced by infra renal aortic cross clamp for 45 min in group B. Before the 45 min ischemia the rabbits in the IPC group underwent four cycles of ischemia preconditioning, i.e.clamping abdominal aorta for 5 min then reperfusing for 5 min. The concentrations of calcium? magnesium? copper? zincum in spinal cord were measured in the 7th day after operation. Postoperative neurologic function and EMG of rear limb, and spinal cord histopathological changes were assessed in all groups after operation. Results The concentration of calcium and copper in spinal cord in group B were significantly higher than those in group A ( P < 0.05 or 0.01 ), but magnesium and zincum significantly lower ( P < 0.05 ). Compared with group C, calcium in group B was significantly higher ( P < 0.01 ), and zincum significantly lower ( P < 0.01 ). Compared with group A or C, the neurologic function and histopatholohical changes were more serious in group B ( P < 0.01 ). And there was significantly worse change of EMG in group B than that in group C ( P < 0.01 ). Conclusions Multi ischemia preconditioning can protect rabbit spinal cord from ischemia reperfusion injury quickly, and one possible cause of its protective effect is to maintain the balance of calcium? magnesium? copper? zincum in ischemic region.
出处
《卒中与神经疾病》
2003年第4期212-215,共4页
Stroke and Nervous Diseases
