双歧杆菌制剂对大鼠肝硬化模型的保护作用

Protection of Bifidobacterium on Rats Model of Cirrhosis

目的 控讨双歧杆菌制剂对大鼠肝硬化模型内毒素血症的保护作用。方法 90只雄性 SD大鼠随机分为正常对照组、预防组、晚期治疗组和模型组。预防组和晚期治疗组分别从不同时期开始给予喂服双歧杆菌制剂。实验第 11周末处死所有大鼠 ,HE染色观察肝脏病理改变 ,测定血清转氨酶、白蛋白水平及血浆内毒素、NO含量。结果 预防组肝硬化病变程度轻 ,晚期治疗组病变程度介于模型组和预防组之间。预防组、晚期治疗组血清 ALT和血浆内毒素、NO水平明显低于模型组 ( P<0 .0 5～ 0 .0 1) ,白蛋白水平高于模型组 ( P<0 .0 5 )。 结论 双歧杆菌制剂通过平衡肠道环境 ,减少了肠源性内毒素血症的发生 ,降低了血浆内毒素水平 。

OBJECTIVE To explore the protection of bifidobacterium on endotoxemia of cirrhotic rats.METHODS 90 male SD rats were divided into 4 groups randomly:normal group preventive group,late treatment group and model group.The preventive group and the late treatment group were given bifidobacterium at different period.At 11 weeks the pathology of livers were observed by HE stain and alanine aminotransferase(ALT),albumin(AL),endotoxin(ET)and nitric oxide(NO)were measured after the rats were sacrificed.RESULTS The pathological process of liver in preventive group and late treatment group were improved.Serum ALT levels and plasma levels of ET and NO were significantly reduced in rats of preventive group and late treatment group compared with those in rats of model group(P<0 05～0 01),and serum AL levels were increased compared with those in rats of model group(P<0 05).CONCLUSION Bifidobacterium could restrain the occurrence of intestinal endotoxemia(IETM) by balancing the intestinal colony.Bifidobacterium may stabilise and remit pathological process of cirrhosis.