尖锐湿疣角质形成细胞凋亡与Fas、Bcl-2及PCNA的表达

Apoptosis and expressions of Fas,Bcl-2 and PCNA of keratinocytes in condyloma acuminatum

目的 :检测尖锐湿疣 (CA)角质形成细胞凋亡和Fas、Bcl 2及PCNA的表达 ,探讨它们在CA发病中的作用及关系。方法 :对 5 1例CA上皮和 18例正常上皮分别采用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记技术检测细胞凋亡 ,采用免疫组化ABC法检测Fas、Bcl 2及PCNA的表达。结果 :CA上皮凋亡指数与正常上皮无明显差异 (P >0 .0 5 ) ,CA上皮Fas、Bcl 2及PCNA指数显著高于正常上皮 (P <0 .0 1) ,Fas表达与Bcl 2表达呈中度正相关 (r=0 .318,P <0 .0 5 )。结论 :CA上皮细胞凋亡与Fas、Bcl 2及PCNA的表达可能与CA的发病有关。

Objective:To investigate the role of apoptosis and expressions of Fas,Bcl 2 and PCNA of keratinocytes in the pathogenesis of condyloma acuminatum (CA),Methods:Apoptosis and expressions of Fas,Bcl 2 and PCNA of keratinocytes were determined by TUNEL and ABC immunohistochemical method in 51 CA and 18 normal epithelium specimens,respectively.Results:The apoptosis index in CA was not obviously different from that in normal specimens( P> 0.05 ).Expressions of Fas,Bcl 2 and PCNA in CA were significantly higher than those in normal samples( P< 0.01),and there was a positive correlation between Fas and Bcl 2 expression ( r =0.318, P< 0.05).Conclusion:Apoptosis and expressions of Fas,Bcl 2 and PCNA of keratinocytes could be involved in the pathogenesis of CA.