2型糖尿病有或无血管合并症患者6种血栓分子标志物的检测与临床意义

Detection of six molecular markers of thrombosis in type 2 diabetes mellitus with or without angiopathy

目的 :了解 2型糖尿病有或无血管合并症患者 6种血栓分子标志物的检测与临床意义。方法 :2例糖尿病患者 90例分为有血管合并症组 (n =5 1)和无合并症组 (n =39)及 30例健康人 (对照组 )均检测血管性假血友病因子(VWF)、血小板α 颗粒膜糖蛋白 14 0 (GMP 14 0 )、纤维蛋白原 (Fbg)、抗凝血酶Ⅲ (AT Ⅲ )、组织纤溶酶原激活物 (t PA)、纤溶酶原激活物抑制物 (PAI)等 6项血栓分子标志物 ,用ELISA和发色底物法检测。结果 :2型糖尿病无论有或无血管合并症的患者的VWF、GMP 14 0、PAI、Fbg均较健康人组高 (P <0 .0 1) ,而有合并症较无合并症组亦显著增高 (P <0 .0 1) ,但t PA相反 ,对照组与无合并症组显著升高 (P <0 .0 1) ;AT Ⅲ在各组之间差异无显著性 (P >0 .0 5 )。结论 :2型糖尿病患者血栓发生与多种因素有关 ,其血管合并症的发生、发展与高凝状态密切相关。

Objective:To observe the clinical significance of six molecular markers of thrombosis in type 2 diabetes mellitus with or without angiopathy.Methods:Six molecular markers of thrombosis including von Willebrand factor(VWF),alpha granule membrane protein 140 (GMP 140),fibrinogen,anti thrombin Ⅲ(AT Ⅲ),tissue plasminogen activator (t PA) and plasminogen activator inhibitor(PAI) were determined by ELISA and colorimetry respectively in 90 patients with type 2 diabetes mellitus (51 with angiopathy and 39 without) and 30 healthy controls.Results:Levels of VWF,GMP 140,fibrinogen and PAI in diabetics and in diabetics with angiopathy were respectively superior to those in controls and in diabetics without angiopathy( P< 0.01),but t PA contents were just the reverse( P< 0.01).There was no significant difference in AT Ⅲ contents among three groups( P> 0.05).Conclusion:Thrombosis in type 2 diabetes mellitus is involved in many factors,and the development and progression of its angiopathy may be related to hypercoagulability.