哮喘患儿血清IL-12、IL-4和总IgE水平的变化、相关及临床意义

Detection of serum IL-12,IL-4 and IgE levels and their clinical significance in asthmatic children

目的 :研究哮喘患儿白细胞介素 12 (IL 12 )、白细胞介素 4 (IL 4 )与总IgE水平的变化、相关及临床意义。方法 :哮喘患儿 30例分为 2组 :(1)发作期组 (n=15 ) ;(2 )缓解期组 (n =15 ) ;以及健康对照组 (n =2 0 )。分别用ELISA法和MEIA法检测血清IL 12、IL4和总IgE水平。结果 :哮喘患儿发作期组血清IL 12水平低于缓解期组 (P <0 .0 5 ) ,缓解期组又显著低于健康对照组 (P <0 .0 1) ;哮喘患儿发作期组血清IL 4水平高于缓解期组 (P <0 .0 5 ) ,缓解期组又显著高于健康对照组 (P <0 .0 1) ;哮喘患儿发作期组血清总IgE水平高于缓解期组 (P <0 .0 5 ) ,缓解期组又显著高于健康对照组 (P<0 .0 1)。哮喘患儿血清IL 12水平与总IgE呈中度负相关 ,哮喘患儿血清IL 4水平与总IgE呈高度正相关。结论 :哮喘患儿血清IL 12水平降低 ,IL 4和总IgE水平升高 ,表明儿童哮喘是一种慢性气道炎症 ,在缓解期仍需抗炎治疗。

Objective:To observe the change of serum IL 12,IL 4 and IgE levels in asthmatic children.Methods:Serum IL 12,IL 4 and IgE contents in 30 asthmatic children and 20 normal controls were determined by ELISA and MEIA technique,respectively.Results:Compared with control group,IL 12 level significantly decreased,and IL 4 and IgE concentrations remarkably increased in asthmatic children( P <0.01),especially in patients with acute stage of asthma.There were a negative correlation between IL 12 and IgE levels and a positive correlation between IL 4 and IgE levels in attack and remission stages of asthma.Conclusion:It is suggested that asthma be a chronic airways inflammation,which requires anti inflammatory therapy even in remission stage.