摘要
目的 :探索不同剂量重组 (酵母 )乙肝疫苗对小鼠细胞免疫调节作用。方法 :将HBsAg免疫Balb/C小鼠一周后取淋巴结制备淋巴细胞悬液 ,经HBsAg刺激培养 4 8h后检测其诱导产生的IL 2、IFN γ的水平 ,另一份细胞同样经HBsAg刺激培养 5 6h后 ,用氚 胸腺嘧啶核苷 (3 H TdR)标记 16h后检测T细胞增殖的水平。用不同剂量HBsAg免疫小鼠后 ,检测血清中抗HBsIgG2a的水平。结果 :中、高剂量组小鼠产生的IL 2、IFN γ、抗HBsIgG2a以及T细胞增殖的水平显著高于低剂量组和对照组。结论 :一定剂量的重组 (酵母 )乙肝疫苗可上调小鼠的细胞免疫功能。
Objective: To study immunoresponse of Balb/C mice with different dose recombinant (yeast) hepatitis B vaccine.Method: After immunized with HBsAg for 7 days,killed the mice and draining lymph nodes were removed and cultured the lymph node cells with 5 μg/ml HBsAg for 48hr, examed the concentration of IL-2 and IFN-γ.On the other hand,cultured the cells for 56hr with HBsAg and labeled with [ 3H]thymidine incorporation for 16hr and examined T cell proliferation. After immunized with different dose HBsAg,examined the lever of IgG2a of anti-HBs of the Balb/C mice.Result: The concentration of IL-2 and IFN-γ,the lever of IgG2a of anti-HBs,T cell proliferation in the group of higher and middle dose HBsAg group were higher than that of control and lower dose group (P< 0.01).Conclusion: higher dose of HBsAg can upregulate celluar immunoresponse in mice.
出处
《中国药师》
CAS
2003年第9期532-534,共3页
China Pharmacist
基金
广东省第 9批科技计划项目基金资助 (编号 :C3 0 40 3 )
